Experimental substantiation of using a synthetic peptide in the complex surgical treatment of acute intestinal obstruction

Abstract

High mortality in the surgical treatment of acute intestinal obstruction (AIO) has warranted the search for new methods of concomitant
therapy. Opioid peptides have proved highly effective in stimulating the lymphatic flow in rat small intestinal mesenteric microvessels, restoring
the impaired microcirculation, and reducing mortality in many diseases. This had determined this study objective: to investigate the role of
lymphatic stimulation in the dynamics of AIO. The study used methods of biomicroscopy, laser Doppler flowmetry, and histological examination
of the mesentery and the small intestinal wall in rats; recording the contractile activity of lymphatic microvessels using photometry; microvessel
morphometry; video and photography. The study results suggested an adaptive role of the lymphatic microcirculation activator (direct-action
opioid-171) in the complex surgical treatment of AIO. A one-time peptide irrigation of the intestinal anastomosis (after removal) and the intestinal injuried by the ligature (before the surgical wound was sutured) increased the survival of animals with AIO by 23%. In all surviving animals, recovery of the intestinal patency, blood and lymphatic microcirculation, and the intestinal wall structure was observed.