Analytical and preparative biosynthesis of S100B recombinant protein using the pBT7-N-His vector system and preliminary studies of structure and function of this protein

  • O. L. Terekhina Institute of General Pathology and Pathophysiology, Moscow, Russia
  • M. K. Nurbekov Institute of General Pathology and Pathophysiology, Moscow, Russia
  • O. P. Dmitrenko Moscow City Tuberculosis Research and Practical Center, Moscow, Russia
  • D. M. Davidov Institute of General Pathology and Pathophysiology, Moscow, Russia
DOI: https://doi.org/10.25557/2310-0435.2018.04.161-164
Keywords: heterologous cDNA expression, recombinant protein S100B, pBT7-N-His vector system, cloning and expression strategy, Genome compiler, protein folding, analytical and preparative protein expression, metal affinity protein chromatography

Abstract

To study structure and functions of the S100B protein in cells and tissues, a series of studies was conducted to optimize the recombinant protein
(recS100B) expression in E. coli. Procedures for analytical expression of recS100B in the pBT7-N-His-S100B03 recombinant plasmid were performed. In SDS-PAGE of bacterial clone lysate, a clear 10 kDa band expression was detected, which was identified as a monomeric form of the protein. Prospects for the S100B study are related with its potential use for investigating molecular mechanisms of PPI interactions in the S100B/RAGE system as a key signal transducer in the cell and body and as a promising object for developing diagnostic systems for monitoring the body state in normal and pathological conditions associated with impaired regulation of the gene and/or functions of the S100B protein.

Published
2018-12-11
How to Cite
Terekhina, O. L., Nurbekov, M. K., Dmitrenko, O. P., & Davidov, D. M. (2018). Analytical and preparative biosynthesis of S100B recombinant protein using the pBT7-N-His vector system and preliminary studies of structure and function of this protein. Patogenez (Pathogenesis), 16(4), 161-164. https://doi.org/10.25557/2310-0435.2018.04.161-164
Issue
Vol. 16 No. 4 (2018): Pathogenesis
Section
Brief reports