Evaluation of the proliferative activity of keratocytes after their culturing with silk fibroin scaffolds
Abstract
The aim of the study was to evaluate changes in the proliferative activity of human corneal keratocytes during co-culturing with biodegradable structures based on silk fibroin containing glial neurotrophic factor.
Materials and methods. The proliferative activity of human corneal keratocytes after culturing with biodegradable constructs based on silk fibroin was investigated in an immunocytochemical study. The Ki-67 protein was used as a marker of cell proliferation.
Results. On the 1st and 3rd days, the activity of Ki-67 protein in the keratocyte culture was not statistically significant. On days 5 and 9, the highest activity of Ki-67 protein in the keratocyte culture was detected with the use of biodegradable constructs containing glial neurotrophic factor at concentrations of 250 ng/ml and 500 ng/ml (p < 0.05).
Conclusion. The use of biodegradable structures based on silk fibroin containing glial neurotrophic factor at concentrations of 250 and 500 ng/ml results in an increase in the proliferative activity of keratocytes.
