Applying of HPLC for S-adenosylhomocysteine and S-adenosylmethionine determination as dysfunction bioindicators of organism

  • B. P. Lusyanin Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. V. Ivanov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • E. D. Virus Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. A. Paltsyn Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • L. M. Kozhevnikova Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. A. Kubatiev Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
Keywords: HPLC, chromatography, S-adenosylhomocysteine, S-adenosylmethionine

Abstract

The article discusses the possibility of using the HPLC method for the determination of S-adenosylhomocysteine (SAH) and S-adenosylmethionine (SАМ) in plasma and tissues using different types of detection. We review and discuss the difficulties encountered in the analysis and their solutions, allowing to achieve the necessary selectivity and sensitivity. The article presents techniques fight against the ion suppression effect in case of the mass spectrometric detection. These approaches are essential for an adequate assessment of the SAH and SАМ content in biological matrices.

Published
2023-03-20
How to Cite
Lusyanin, B. P., Ivanov, A. V., Virus, E. D., Paltsyn, A. A., Kozhevnikova, L. M., & Kubatiev, A. A. (2023). Applying of HPLC for S-adenosylhomocysteine and S-adenosylmethionine determination as dysfunction bioindicators of organism. Patogenez (Pathogenesis), 12(1), 15-20. Retrieved from https://pathogenesis.pro/index.php/pathogenesis/article/view/635
Issue
Vol. 12 No. 1 (2014): Патогенез
Section
Reviews