Effects of simulated microgravity on cell cycle and viability of human endothelial-like EA.hy 926 cells and human neuroblastoma SHSY-5Y cells

Abstract

The results of studies of the cell cycle under microgravity are often contradictory, and depend on many factors, such as cell type, gravity conditions, exposure time and methods. In this study, the influence of simuiated microgravity on cell cycle and viability of human endotheiial-like EA.hy 926 cells and human neuroblastoma SHSY-5Y cells was observed. We anaiyzed changes in the cell cycles after the cells exposure to simuiated microgravity using a Deskiop Random Positioning Machine (RPM). Cell cycles deiermined by flow cytometry showed, that percentage of EA.hy 926 cells in the G0/G1-phase alter 24 and 96 h of RPM-simulated microgravity was significantly increased as compared to the control group, however, after 120 h of RPM-simulated microgravity, the difference was not significant. In contrast, the percentage of neuroblastoma SHSY-5Y cells in the cell cycles was no significant difference after 24 and 96 h of RPM-simulated microgravity. But the percentage of SHSY-5Y cells increased in the G0/G1-phase and decreased in the S-phase by comparison with the control group alter 120 h of RPM-simulated microgravity. Thus, simulated microgravity inhibits the cell cycle progression of human EA.hy 926 and neuroblastoma SHSY-5Y cells from the G0/G1 to the S-phase. In the present study we showed that microgravity inhibits cell-cycle progression in EA.hy 926 endoheiial cells at the eariier stages as compared to human SHSY-5Y neuroblastoma cells. We found that the adverse effects of simuiated microgravity have different impacts and depend on the cell-type of cells.