Determination of bound cysteine, homocysteine and cysteinylglycine fraction in blood plasma by capillary electrophoresis

  • A. V. Ivanov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • B. P. Luzyanin Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • E. D. Virus Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. C. Rybakov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. A. Kubatiev Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
Keywords: homocysteine, cysteine, cysteinylglycine, capillary electrophoresis, thiocarbonyldiimidazole

Abstract

The potential of capillary electrophoresis with thiocarbonydiimidazole derivatization to detection of protein-bound aminothiols in blood plasma was investigated. The electrokinetic concentration of aminothiols derivates allows to improve significantly the detection limit in comparison with hydrodynamic injection, but its effectiveness is limited by salts content in sample. The proposed method makes possible the quantitative determination of protein-bound aminothiols (cysteine, homocysteine and cysteinylglycine) in blood plasma with sufficient sensitivity (about of 1 mkM of homocysteine).

Published
2023-05-17
How to Cite
Ivanov, A. V., Luzyanin, B. P., Virus, E. D., Rybakov, A. C., & Kubatiev, A. A. (2023). Determination of bound cysteine, homocysteine and cysteinylglycine fraction in blood plasma by capillary electrophoresis. Patogenez (Pathogenesis), 11(4), 49-53. Retrieved from https://pathogenesis.pro/index.php/pathogenesis/article/view/652
Issue
Vol. 11 No. 4 (2013): Патогенез
Section
Original investigations