Study of the regenerative potential of placental cells in model experiments on mice
Abstract
A placenta may be a source of both mesenchymal and hematopoietic stem cells. The human placenta is widely used in cosmetology but its properties are not quite clear in light of its possible application for a transplantation in the regenerative medicine. The aim of this report was to study regularities of engraftment of an entire cellular placenta fraction transplanted to an adult organism in the model experiments on mice of the C57BL/6 strain. Methods. The placental suspension obtained from fetuses of the 18—20 days of pregnancy was used. One of their parents carried the Tg(ACTB-EGFP)1Osb/J green fluorescent protein gene. The 4 to 6 months old C57BL/6 mice without this transgene and intact or irradiated in the sublethal dose were used as recipients. The suspension was transplanted intravenously or intraperitoneally, the second route of administration was being preferable as much more safe. Results. The using of fluorescent microscopy showed the exocolony formation in a spleen and the presence of the negligible quantity of the luminous cells only in the bone marrow of the irradiated mice starting with the 11th irradiation day. It was shown that the donor cells were appeared in the recipient organism only in the case when the placentas of fetuses from the females carrying GFP gene were used. An analysis of the placenta cell suspension on CD45 and CD117 markers revealed among them the presence of the hematopoietic stem cells as well as leucocytes which were their differentiated progenies. Conclusion. Results obtained in this work may be used in future to develop the methods of therapy and life prolongation of the delivered women who reserved their own placenta by cryopreservation using their own cellular substance.
