Silver nanoparticle synthesis and an estimation of these effects in vivo on the peritoneal cells from the male mouse C57BL/6

  • A. G. Filippov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. P. Kaplun Lomonosov Moscow State Academy of Fine Chemical Technology, Moscow, Russian Federation
  • A. A. Kubatiev Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
Keywords: silver nanoparticles, toxicity, neutrophils, lymphocytes, macrophages, tannins, polyphenols, nanomedicine

Abstract

The silver nanoparticles (Ag-NPs) have been prepared using the tannins or Quercus cortex as the reducing and stabilizing agents as well as the phosphatidyl choline as a co-stabilizer. The Ag-NPs forms and sizes were evaluated using a transmission electron microscopy. Ag-NPs disperses electron specters were analyzed by spectrophotometer. In vivo biological effects of these Ag-NPs were estimated using the male mouse C57Bl/6. The Ag-NPs [10 — 500 microM] have been injected intraperitoneally for 3 hours followed by a quantitative analysis of the peritoneal cells using the flow cytometer. The dose-dependent effects of these Ag-NPs have been registered. The peritoneal neutrophils have been found to be the most sensitive cells compared with macrophages and lymphocytes. The Ag-NPs in the concentration < 10 microM possess the immune modulatory effects and were not toxic that allows us to use these Ag-NPs in the future work with the biological systems.

Published
2023-05-17
How to Cite
Filippov, A. G., Kaplun, A. P., & Kubatiev, A. A. (2023). Silver nanoparticle synthesis and an estimation of these effects in vivo on the peritoneal cells from the male mouse C57BL/6. Patogenez (Pathogenesis), 11(4), 59-63. Retrieved from https://pathogenesis.pro/index.php/pathogenesis/article/view/654
Issue
Vol. 11 No. 4 (2013): Патогенез
Section
Original investigations