Silver nanoparticle synthesis and an estimation of these effects in vivo on the peritoneal cells from the male mouse C57BL/6
Keywords:
silver nanoparticles, toxicity, neutrophils, lymphocytes, macrophages, tannins, polyphenols, nanomedicineAbstract
The silver nanoparticles (Ag-NPs) have been prepared using the tannins or Quercus cortex as the reducing and stabilizing agents as well as the phosphatidyl choline as a co-stabilizer. The Ag-NPs forms and sizes were evaluated using a transmission electron microscopy. Ag-NPs disperses electron specters were analyzed by spectrophotometer. In vivo biological effects of these Ag-NPs were estimated using the male mouse C57Bl/6. The Ag-NPs [10 — 500 microM] have been injected intraperitoneally for 3 hours followed by a quantitative analysis of the peritoneal cells using the flow cytometer. The dose-dependent effects of these Ag-NPs have been registered. The peritoneal neutrophils have been found to be the most sensitive cells compared with macrophages and lymphocytes. The Ag-NPs in the concentration < 10 microM possess the immune modulatory effects and were not toxic that allows us to use these Ag-NPs in the future work with the biological systems.
