Determination of bound cysteine, homocysteine and cysteinylglycine fraction in blood plasma by capillary electrophoresis

Authors

  • A. V. Ivanov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • B. P. Luzyanin Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • E. D. Virus Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. C. Rybakov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • A. A. Kubatiev Institute of General Pathology and Pathophysiology, Moscow, Russian Federation

Keywords:

homocysteine, cysteine, cysteinylglycine, capillary electrophoresis, thiocarbonyldiimidazole

Abstract

The potential of capillary electrophoresis with thiocarbonydiimidazole derivatization to detection of protein-bound aminothiols in blood plasma was investigated. The electrokinetic concentration of aminothiols derivates allows to improve significantly the detection limit in comparison with hydrodynamic injection, but its effectiveness is limited by salts content in sample. The proposed method makes possible the quantitative determination of protein-bound aminothiols (cysteine, homocysteine and cysteinylglycine) in blood plasma with sufficient sensitivity (about of 1 mkM of homocysteine).

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Published

2023-05-17

Issue

Vol. 11 No. 4 (2013): Патогенез

Section

Original investigations

How to Cite

Determination of bound cysteine, homocysteine and cysteinylglycine fraction in blood plasma by capillary electrophoresis. (2023). Patogenez (Pathogenesis), 11(4), 49-53. https://pathogenesis.pro/index.php/pathogenesis/article/view/652

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