Lipid peroxidation in rodents with exudative inflammation

Abstract

Aim. To study levels of conjugated dienes (CD), primary products of lipid peroxidation, and malondialdehyde (MDA), a secondary product of lipid peroxidation, in peritoneal exudate and serum of rats and mice with exudative inflammation. Materials and methods. CD and MDA concentrations were measured spectrophotometrically in peritoneal exudate from mice with glycogen-induced peritonitis and rats with acetic acid-induced peritonitis and in serum of rats with acetic-induced peritonitis and carrageenan-induced paw edema. Results. MDA concentrations were increased in the peritoneal exudate of rats with acetic acid-induced peritonitis and mice with glycogen-induced peritonitis as compared to intact animals. Both MDA and CD concentrations were increased in the serum of rats with acetic acid-induced peritonitis at 3 hours of the inflammation induction. However, only MDA levels significantly increased in rats with carrageenan-induced paw edema at 5 hours of the phlogogen injection, whereas CD levels did not differ from intact animals. Conclusion. These results might be useful for further investigation of changes in primary and secondary products of lipid peroxidation in experimental inflammatory conditions to characterize their pathogenesis and to study effects of antioxidative and anti-inflammatory compounds on concentrations of lipid peroxidation products.