Culture of human and animal cells: isolation, cultivation, cryopreservation and control

Abstract

The progress in modern biology, immunology and medicine is largely due to the creation of conditions for in vitro
cultivation of different origin cells, to the discovery of stem and progenitor cells, and the high prospect of their
application in research and production of preparates for immunization, diagnosis and treatment. The presence of
several cell cultures in the laboratory, especially dealing with obtaining their own cell culture, a prerequisite not
only for cell contamination by foreign microorganisms but contamination by cells of other cell lines. These findings
underscore the importance of creation of specialized cell culture collections and characterization of incoming and
newly produced cell cultures used in research or in applied research. The stability of the production of drugs for
immunization, diagnosis or treatment largely depends on the stability and biosafety of used cells. Standardized
and certified cell culture is the basis for producing a safe product. Creation a system of well characterized and
controlled cell collections and certified cell banks suitable for research, development of new production technologies
for medicines and treatment will ensure quality assurance, stability and security cells. In this review current
approaches and requirements for control of cell cultures, the importance of the creation and control of seed and
working cell banks used in scientific research and production, diagnosis and treatment are considered.