Identifying pathogenetically important indicators of microbiome in chronic endometritis in women with reproductive disorders

Abstract

Despite multiple studies of the pathogenesis of chronic endometritis, there are still no unambiguous methods for assessing the status and prognosis of this disease. A relevant objective is to identify the most significant markers for changes in the microbiome during chronic endometritis based on a comprehensive evaluation of different microbiocenoses as an important component of immune resistance to unfavorable factors. The aim of this study was to develop pathogenetically substantiated microbiological criteria for microbiome disturbances during chronic endometritis in women with reproductive disorders using methods of discriminant analysis. Methods. The study used microbiological, molecular genetic and statistical methods. Results. The discriminant analysis allowed to identify the most informative indicators of microbiome changes: Corynebacterium spp. (vaginal biotope), CNS (vaginal biotope), serine proteinase gene (Spr E), Neisseria spp. (nasopharyngeal biotope), Streptococcus β-hemolytic (nasopharyngeal biotope), Lactobacterium spp. (vaginal biotope), Enterococcus faecalis (intestinal biotope), and fungi of the genus Candida (vaginal biotope). The least informative indicators were fungi of the genus Candida (intestinal biotope), and Enterococcus faecium (nasopharyngeal biotope). Conclusion. Identifying the most informative indices of the microbiome of various biotopes makes it possible to construct a mathematical model for pathogenetic substantiation of methods, which could be used for correction of microbiome changes in women with chronic endometritis and reproductive disorders.