Влияние релиз-активных антител к гистамину на функциональную активность H4-рецепторов in vitro

P. L. Naplyokova; A. G. Emelyanova; S. A. Tarasov; E. N. Kozhevnikova; S. G. Morozov

doi:10.25557/2310-0435.2018.03.135-137

Effect of release-active antibodies on the in vitro functional activity of H4-receptors

P. L. Naplyokova LLC NPF Materia Medica Holding, Moscow, Russia
A. G. Emelyanova Institute of General Pathology and Pathophysiology, Moscow, Russia
S. A. Tarasov Institute of General Pathology and Pathophysiology, Moscow, Russia
E. N. Kozhevnikova Institute of General Pathology and Pathophysiology, Moscow, Russia
S. G. Morozov Institute of General Pathology and Pathophysiology, Moscow, Russia

DOI: https://doi.org/10.25557/2310-0435.2018.03.135-137

Keywords: histamine, released-active antibodies, allergy, H4-receptor, GTPγS assay

Abstract

The aim of the study was to evaluate the effect of released-active antibodies (RA Abs) to histamine on the functional state of H₄-receptor using the GTPγS assay in vitro. Experiments were performed on CHO-K1 cell membranes expressing the human recombinant H₄-receptor. The functional activity of H₄-receptor was assessed by the amount of GTPγ[³⁵S] bound to the membranes. It was shown that RA Abs to histamine were antagonistic to H₄-receptors.

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Published

2018-12-31

How to Cite

Naplyokova, P. L., Emelyanova, A. G., Tarasov, S. A., Kozhevnikova, E. N., & Morozov, S. G. (2018). Effect of release-active antibodies on the in vitro functional activity of H4-receptors. Patogenez (Pathogenesis), 16(3), 135-137. https://doi.org/10.25557/2310-0435.2018.03.135-137