Гликирование альбумина in vitro при нормальной и повышенной концентрации глюкозы

N. Yu. Lotosh; S. V. Savelyev; A. A. Selishcheva

N. Yu. Lotosh ANO «Institute of Biomedical Problems», Moscow, Novodanilovskaya nab., 4a
S. V. Savelyev Institute of Human Morphology, 117418, Moscow, st. Cjurupy, 3
A. A. Selishcheva Lomonosov Moscow State University, 119991, Russia, Moscow, Lenin Hills, 1, page 12, MSU

Keywords: high performance liquid chromatography, ketoamine, mercaptalbumin, non-mercaptalbumin, insulin- dependent diabetes, human serum albumin

Abstract

Albumin — the main plasma protein. In blood it exposed to the reactions of glycation and oxidation. Susceptible
to oxidation as the different amino acid residues in the molecule of albumin and the free SH-group of Cys at position
34. In diabetes, glycation and oxidation of albumin occurs to a greater degree than normal. The present study investigates
the effect of oxidation of SH-groups in the molecule of human serum albumin in its glycation at normal and
high values of glucose (5 mM and 50 mM). We found that Trp 214 fluorescence quenching takes place only when
the ketoamine level in molecule of albumin level is characterized for chronic hyperglycemia (above 0.4 M/M). Also
found that albumin is glycated largely if thiol pool albumin is reduced, i.e. SH-groups are partially oxidized.

Glycation of albumin in vitro at normal and elevated blood glucose

Abstract