Study of cell therapy possibilities for renewal of feminine sexual function on mouse model experiments

Abstract

Oncoming menopause in woman is accompanying by severe breakdown of her health and performance capability. Bone marrow (BM) transplantation might be a way to postpone aging alterations in woman’s organism accompanying by serious pathologies. To test this method the model experiments have been carried out in which the female mice of 6—8 months of age and C57BL/6J inbred strain were used as recipients and the female C57BL/6 — Tg(ACTB-EGFP)1Osb/J mice of 1,5—2 months of age were used as donors. BM infusions were carried out once every three months. During the posttransplantation period the number of delivered litters and the number of survived pups and mean lifespan (MLS) were defined in the recipient females. In addition, a chance of offspring delivery with the gene of green fluorescent protein by recipients (namely, transformation of BM stem cells into germ cell line) was examined. It was found that the experimental animals lived longer that the control ones. They delivered more litters and more their offsprings per litter survived as compared with the control animals. Pups with EGFP gene were not found between offspring of the BM recipients. It is concluded that the direct relationship exists between increasing fertility data and MLS. The transformation of the BM stem ñells into the germ line cells was not detected. On basis of our data the transplantation of the own BM obtained from women of young age and preservated in a cryobank is proposed as a method of saving and maintaining woman’s sex.