Effects and mechanisms of pegylated hyaluronidaze in C57BL/6 mice under bleomycin induced pulmonary fibrosis

  • E G Skurihin FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • O V Pershina FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • V A Krupin FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • A M Reztcova FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • N N Ermakova FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • L A Ermolaeva FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • E S Khmelevskaya FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • A V Pakhomova FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • E S Pan FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
  • P G Madonov State Educational Institution of Higher Professional Education Novosibirsk State Medical University of the Russian Health Ministry
  • M A Shilova State Educational Institution of Higher Professional Education Novosibirsk State Medical University of the Russian Health Ministry
  • A M Dygay FSBSI «Research Institute of Pharmacology and Regenerative Medicine named after E.D.Goldberg», 634028, Tomsk, Lenin Avenue, 3
Keywords: pegylated hyaluronidase, hematopoietic stem cells, hematopoietic progenitor cells, mesenchymal

Abstract

The effects of pegylated hyaluronidase (pegGD) were studied in mice C57BL/6 under the bleomycin injury of alveolar epithelium. pegGD is a testicular hyaluronidase, which had been modified by activated polyethylene oxide using electron-beam synthesis (LTD «Scientific Future Management», Novosibirsk). pegGD has a little effect on the development of inflammatory response in lungs, reduces the area of connective tissue in bleomycin lungs, the level of hyaluronic acid, hydroxyproline and general collagen is decreased in lung homogenates under the treatment. Antifibrotic effect of pegGD is observed under prophylactic and therapeutic mode of administration and is more pronounced than GD has. pegGD reduces the number of hematopoietic stem and progenitor cells in the lung parenchyma of sick animals, while the number of mesenchymal stem cells is increased. The reduction of clonal activity of pulmonary progenitor fibroblast cells is recorded in the same time. Thus, pegGD is a potential antifibrotic agent for the prevention and treatment of toxic lung fibrosis.

Published
2015-09-30
How to Cite
Skurihin, E. G., Pershina, O. V., Krupin, V. A., Reztcova, A. M., Ermakova, N. N., Ermolaeva, L. A., Khmelevskaya, E. S., Pakhomova, A. V., Pan, E. S., Madonov, P. G., Shilova, M. A., & Dygay, A. M. (2015). Effects and mechanisms of pegylated hyaluronidaze in C57BL/6 mice under bleomycin induced pulmonary fibrosis. Patogenez (Pathogenesis), 13(3), 56-64. Retrieved from https://pathogenesis.pro/index.php/pathogenesis/article/view/35
Issue
Vol. 13 No. 3 (2015): Pathogenesis
Section
Original investigations