Superoxide anion releasing by neutrophils from young adult atopic dermatitis patients

Abstract

The aim if this work was the investigation of superoxide anion generation by purified blood neutrophils obtained from young adult men with atopic dermatitis. Both, N-formyl-methionyl-leucyl-phenylalanine (fMLP) and receptor independent stimulation by phorbol myristate acetate (PMA) were used for NADPH oxidase stimulation and superoxide anion production. Both, fMLP and PMA have been found to slightly increase the superoxide production by neutrophils at the initial period of atopic dermatitis relapse (if index SCORAD was less then 20 points) compared with healthy donors. The superoxide anion generation by neutrophils have been found to be depressed at the moderate atopic dermatitis compared with donors and the mild atopic dermatitis patients. Also, the dynamic of neutrophil activity has been measured during three hours after breakfast feeding. The curve of this activity was considerably different between healthy donors and atopic dermatitis patients. At the moderate atopic dermatitis, superoxide production has been shown to be significantly suppressed compared with healthy donors and atopic patients with mild case of disease.