Инфламэйджинг: роль в патогенезе и молекулярной диагностике возраст-ассоциированной дилатационной кардиомиопатии

I. M. Kvetnoy; N. S. Linkova; A. E. Pykhalaskaya; K. L. Kozlov; G. I. Gurko; M. A. Paltsev

I. M. Kvetnoy St. Petersburg State Research Institute of Phthisiopulmonology, St. Petersburg, Russia; St. Petersburg State University, St. Petersburg, Russia http://orcid.org/0000-0001-7302-5581
N. S. Linkova Research Center, St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia; Academy of Postgraduate Education of the Federal Scientific and Clinical Center of Specialized Medical Assistance and Medical Technologies of the Federal Biomedical Agency of Russia, Moscow, Russia; Belgorod State University, Belgorod, Russia http://orcid.org/0000-0001-5156-5421
A. E. Pykhalaskaya Research Center, St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia http://orcid.org/0000-0001-5401-414X
K. L. Kozlov Research Center, St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia http://orcid.org/0000-0003-3660-5864
G. I. Gurko Research Center, St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia http://orcid.org/0000-0001-9725-6253
M. A. Paltsev M.V. Lomonosov Moscow State University, Moscow, Russia http://orcid.org/0000-0002-1469-4934

Keywords: dilated cardiomyopathy, inflammaging, molecular diagnostics, saliva, aging

Abstract

Background. Inflammaging and disorder of melatonin synthesis in the pineal gland play an important role in the pathogenesis of dilated cardiomyopathy (DC) in elderly and old people. An important objective of molecular medicine is searching for signal molecules, markers of DC.
The aim of this work was to compare concentrations of IL-1β, IL-6, Klotho, Sirt3, FGF23, and melatonin in saliva of elderly and old DC patients and persons without DC.
Methods. Concentrations of signal molecules were measured in saliva by ELISA.
Results. Salivary concentrations of inflammaging markers (IL-1β, IL-6) in elderly and old DC patients were 8.6-19.1 times higher than in persons without DC. Salivary concentration of Klotho protein, an inhibitor of pro-inflammatory cytokine synthesis, was increased 4.2-7.6 times in elderly and old DC patients compared to the values in persons without DC. Salivary concentrations of melatonin and melatonin-regulated Sirt3 were decreased 2.1-4.3 times in elderly and old DC patients compared to the values in persons without DC. Salivary concentration of FGF23 did not depend on the age or presence of DC.
Conclusion. Inflammaging plays in important role in the pathogenesis of DC in elderly and old people as evidenced by decreased synthesis of Klotho and increased levels of the pro-inflammatory cytokines IL-1β and IL-6. Another step in the DC pathogenesis is decreased melatonin synthesis and the resultant dysregulation of the Mst1/Sirt3 signaling way. Measuring concentrations of IL-1β, IL-6, Klotho, SIRT3, and melatonin in saliva can be used for molecular diagnostics of DC in elderly and old people.

Inflammaging: Role in pathogenesis and molecular diagnostics of age-related dilated cardiomyopathy

Abstract