Research of angiogenic potential of human multipotent mesenchymal stromal cells

  • I. N. Saburina Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; Russian Medical Academy of Continuing Vocational Education, Moscow, Russian Federation
  • A. A. Gorkun Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • I. M. Zurina Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
  • N. V. Kosheleva Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; M.V. Lomonosov Moscow State University, Moscow; Russian Federation
  • V. S. Repin Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; Russian Medical Academy of Continuing Vocational Education, Moscow, Russian Federation
Keywords: multipotent mesenchymal stromal cells, angiogenesis, endothelial progenitor cells, Matrigel

Abstract

To study the angiogenic potential of cell cultures a new 3D model of using the VEGF-induced spheroids from umbilical cord multipotent mesenchymal stromal cells (UC MMSC) was elaborated. It was shown that in the process o f forming spheroids UC MMSC the number of early endothelial progenitor cells expressing CD34+, CD133+ increased as compared with 2D culture. The expression of markers of late endothelial progenitor cells (CD31+/vWF+/ F lk -1+/ VEGF+) was induced by VEGF. After transfer into Matrigel in spheroids the formation tubular like structures were discovered.

Published
2023-05-26
How to Cite
Saburina, I. N., Gorkun, A. A., Zurina, I. M., Kosheleva, N. V., & Repin, V. S. (2023). Research of angiogenic potential of human multipotent mesenchymal stromal cells. Patogenez (Pathogenesis), 11(1), 59-62. Retrieved from https://pathogenesis.pro/index.php/pathogenesis/article/view/685
Issue
Vol. 11 No. 1 (2013): Патогенез
Section
Original investigations