Effect of pentylenetetrazole on the GABAA-coupled Сl–, НСОз– -activated Мg2+-ATPase activity of the plasma membrane from rat brain both in vitro and in vivo experiences

Abstract

Effect of the pentylenetetrazole (PTZ) on the couplied with GABAa-receptor Сl^–, НСО_з^–-activated Мg²⁺-ATPase of the plasma membranes from rat brain it was investigated both experiences in vitro and in vivo. It was found in vitro that pentylenetetrazole in range conceniraiions 15–20 μM enhances the activity of the «basal» Мg²⁺-ATPase and eliminated the activating effect of Сl^– and Сl^– + НСО_з^– on the enzyme. Similarly, the effect of the PTZ on the activity of the «basal» Мg²⁺-ATPase activity and it is activating by anions it was observed under intraperitoneal injection of the it at dose 65 mg/kg. It was conclusion about involved of the coupled with GABA_A-receptor Сl^–, НСО_з^– -activated Мg²⁺-ATPase of the neuronal membrane under synaptic transmission of the CNS and pathogenesis of the PTZ-induced seizures.