The effect of calendula polysaccharides on erythropoiesis in animals with induced stress

Abstract

Purpose. To study the effect of polysaccharides of calendula inflorescences on the erythropoiesis process in rats, on models of immobilization stress.

Research methods. The study was conducted on male Wistar rats (n = 24) divided into three equal groups: I – control, II – stress, III - stress after five days of intraperitoneal administration of calendula inflorescence polysaccharides. Animals of all groups were kept in standard vivarium conditions. The immobilization stress model was created by placing animals in a restrain for 48 hours without food and water. At the end of the experiment, blood and femoral bone samples were taken for further research. Тhe number of red blood cells and hemoglobin in the blood were determined, as well as changes in the types and number of erythroblastic islets of the femoral bone marrow.

Results. It was found that the preliminary administration of polysaccharides from calendula inflorescences reduces the intensity of erythropoiesis response to stress, limiting the number of class 1 erythroblastic islets and increasing the number of peripheral blood erythrocytes due to the mobilization of young forms of erythrocytes from the bone marrow into the bloodstream. Preventive administration of polysaccharide is also associated with the absence of a significant increase in the level of hemoglobin in the blood, characteristic of a physiological response to stress, but is accompanied by a significant decrease in its concentration in red blood cells, as well as a decrease in the volume of the latter.

Conclusion. Preliminary intraperitoneal administration of calendula inflorescence polysaccharides significantly modifies the adaptive responses of the bone marrow to immobilization stress in rats, which is manifested by significantly less intense activation of erythropoiesis and increased mobilization of young forms of erythrocytes into the bloodstream.