Comparison of chemotaxis properties and expression of neutrophil receptor in patients with obesity and normal weight after abdominoplasty operation

Abstract

Abdominoplasty is performed with an aim of body aesthetic correction, therefore, the presence of postoperative complications is highly undesirable. Obesity (BMI >30 kg/m²) is a risk factor for development of postoperative complications, due to the active role of adipose tissue in the inflammatory process, in which neutrophils play a significant role. Aim of our work was to study receptor expression of the blood neutrophils as well as the inflammatory cytokine levels in blood serum obtained from patients with normal weight (n = 22) and obesity (n = 36) after the abdominoplasty. Methods. The inflammatory marker level in the blood serum was determined by ELISA. The neutrophils were stained with fluoresceine-conjugated monoclonal antibodies and the receptor expression was analyzed by flow cytometry. Results. Performed abdominoplasty in persons with obesity determined the elevated serum level of proinflammatory cytokines IL-6, IL-34, TNF-α compared to patients with normal body weight. Patients with obesity had significantly higher expression of receptors CD66b, CD63, CD11b, CD85a, TLR-2, TNFR1, TNFR2, as well as chemokines receptors FPR1, FPR2 and CXCR2 on neutro phils compared to patients with normal body weight. Conclusions. The detected changes in the activity of blood neutrophils in obese patients may have the pathophysiological significance for the development of operating scar inflammation as well as serve as an additional risk factor for complications after abdominoplasty.