Прямое влияние гранулоцитарно-макрофагального колониестимулирующего фактора (GM-CSF) на функциональные свойства Т-лимфоцитов человека

N. D. Gazatova; O. B. Melashchenko; M. E. Meniailo; V. A. Shmarov; V. V. Malashchenko; E. V. Morozova; A. G. Goncharov; V. I. Seledtsov

doi:10.25557/2310-0435.2018.02.37-42

N. D. Gazatova Immanuel Kant Baltic Federal University, Kaliningrad, Russia http://orcid.org/0000-0002-4646-3436
O. B. Melashchenko Immanuel Kant Baltic Federal University, Kaliningrad, Russia http://orcid.org/0000-0002-0892-1168
M. E. Meniailo Immanuel Kant Baltic Federal University, Kaliningrad, Russia http://orcid.org/0000-0003-4630-4934
V. A. Shmarov Immanuel Kant Baltic Federal University, Kaliningrad, Russia http://orcid.org/0000-0001-7156-3415
V. V. Malashchenko Immanuel Kant Baltic Federal University, Kaliningrad, Russia http://orcid.org/0000-0003-3437-0926
E. V. Morozova Immanuel Kant Baltic Federal University, Kaliningrad, Russia
A. G. Goncharov Immanuel Kant Baltic Federal University, Kaliningrad, Russia
V. I. Seledtsov Immanuel Kant Baltic Federal University, Kaliningrad, Russia

DOI: https://doi.org/10.25557/2310-0435.2018.02.37-42

Keywords: granulocyte-macrophage colony-stimulating factor, T cell, adaptive immunity, CD25, CD38, interleukin, interferon

Abstract

Background. We studied direct effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the function of T-lymphocyte subpopulations. Methods. CD3⁺ T cells were isolated from the blood of healthy donors by positive magnetic separation. Isolated T cells were activated by particles conjugated with antibodies (Abs) to human CD3, CD28, and CD2 molecules. Membrane expression of CD4, CD45RA, CD197, CD25, and CD38 was evaluated by flow cytofluorometry. The contents of interferon-γ (IFN-γ), interleukin-2 (IL-2), IL-4, and IL-10 were determined in culture supernatants by the enzyme immunoassay. Results. GM-CSF at concentrations of 0.01—10.0 ng/ml had no significant impact on the content of CD25⁺ cells among activated T lymphocytes. At the same time, GM-CSF at 0.1—1.0 ng/ml was able to noticeably increase the CD38⁺ cell content among both naive CD45RA⁺/CD197⁺ T cells and central memory CD45RA^-/CD197⁺ T cells, without significantly influencing the CD38 expression on effector CD45RA^-/CD197^- and terminal-differentiated CD45RA⁺/ CD197^- effector T cells. GM-CSF at a relatively low concentration (0.01 ng/ml) significantly decreased T-cell production of INF-γ whereas GM-CSF at a high concentration (10.0 ng/ml) detectably enhanced secretion of IL-2 and IL-4 and lowered IL-10 production. Conclusion. The results suggest that direct effects of GM-CSF on the T cell function could be largely determined by both its belonging to a subpopulation and the cytokine concentration in the cell microenvironment.

Direct effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on functional features of human T-lymphocytes

Abstract