Диагностическое значение группы генов микроРНК, гиперметилированных при немелкоклеточном раке лёгкого

V. I. Loginov; S. V. Rykov; D. S. Khodyrev; T. P. Kazubskaya; E. A. Braga

doi:10.25557/2310-0435.2018.03.112-115

V. I. Loginov Institute of General Pathology and Pathophysiology, Moscow, Russia; Medical Genetics Research Center, Moscow, Russia http://orcid.org/0000-0003-2668-8096
S. V. Rykov State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center «Kurchatov Institute», Moscow, Russia http://orcid.org/0000-0002-0485-755X
D. S. Khodyrev Federal Scientific and Clinical Center of Specialized Medical Assistance and Medical Technologies of the Federal Biomedical Agency of Russia, Moscow, Russia
T. P. Kazubskaya N.N. Blokhin National Medical Research Center of Oncology, Moscow, Russia
E. A. Braga Institute of General Pathology and Pathophysiology, Moscow, Russia; Medical Genetics Research Center, Moscow, Russia

DOI: https://doi.org/10.25557/2310-0435.2018.03.112-115

Keywords: lung cancer, miRNA gene methylation, ROC analysis, diagnostic marker systems, sensitivity, specificity, AUC

Abstract

The high mortality in non-small cell lung cancer (NSCLC) and the lack of effective diagnostics indicate a need for new NSCLC biomarkers. Epigenomic studies have shown that the proportion of hypermethylated miRNA genes is several times higher than of protein-coding genes, which makes them promising markers. The aim of the study was to evaluate diagnostic characteristics of a group of hypermethylated miRNA genes in NSCLC and its subtypes, squamous cell lung cancer (SLC), and adenocarcinoma (AC). On a set of 39 NSCLC samples and 20 samples of lung tissue from «donors» (dead from non-oncological disease), methylation-specific PCR was used to perform a comparative analysis of methylation of a group of genes, MIR-125B-1, MIR-129-2, MIR-137, and MIR -375. The ROC-analysis identified 6 potential diagnostic systems of markers for NSCLC, SLC, and AC, which had high sensitivity (85—92%) and specificity (90—95%) and the AUC value of 0.89—0.94. The diagnostic systems for SLC and NSCLC showed the best values.

Diagnostic value of a group of miRNA genes hypermethylated in non-small cell lung cancer

Abstract