Anti-inflammatory activity of released-active antibodies to interferon-gamma, CD4-receptor, and histamine against respiratory-syncytial viral infection

Abstract

Background. Respiratory syncytial virus (RSV) causes the most dangerous infections in children, especially those under one year, being the main cause of deaths and contributing to the development of bronchial asthma. There is no effective treatment for the causative infection, and preventive measures are limited. The use of drugs based on released-active antibodies (RA Abs) that target the immune response may be promising. Aim. The aim of the work was to study preventive effects of RA Abs to interferon-gamma (IFN-gamma), CD4 receptor, and histamine on RSV infection in vivo. Methods. Balb /c mice were infected with RSV intranasally at a dose of 5 × 106 TCID50 per mouse. For 5 days prior to infection, RA Abs to IFN-gamma, CD4 receptor, and histamine or the negative control (purified water) were intragastrically administered to the animals. Infiltration of inflammatory cells into the respiratory tract was evaluated 6 days after infection. Results. RA Abs to IFN-gamma, CD4 receptor, and histamine significantly (p < 0.05) reduced the total infiltration of inflammatory cells into the lungs, as well as levels of lymphocytes and macrophages compared with the control groups. Conclusion. The prophylactic use of RA Abs to IFN-gamma, CD4 receptor, and histamine helps to alleviate severity of airway inflammation in experimental animals infected with RSV.