Study of biomarkers for damage of renal tissue in patients with oxalate urolithiasis after transurethral contact ureterolithotripsy

Abstract

Identification of markers for renal pathology and implementation of biochemical indices objectively reflecting the development of pathological process in urolithiasis disease would significantly facilitate detection of this disease and contribute to development of proper and adequate treatments in oxalic urolithiasis. Increased lipid peroxidation products are known to be present in urolithiasis. Therefore, detection of lipid peroxidation metabolites in serum could serve as a criterion for course and outcome of various forms of this disease. Materials and methods. Concentrations of urinary lipocalin (NGAL), plasma malonic dialdehyde (MDA) and diene conjugates (DC), and activities of catalase and glutathione peroxidase (GPO) were measured in patients with oxalate urolithiasis after transurethral contact ureterolithotrization (CULT). The study included 72 patients; 42 of them underwent CULT and 30 patients had documented oxalate urolithiasis without surgical treatment. Control group consisted of 10 healthy subjects. Patients were divided into groups based of the stage of treatment, and blood samples were collected. Results. Concentrations of MDA and DK and GPO activity were increased during the surgery and first postoperative days compared to the
control group. Catalase activity and urinary concentration of the NGAL stress peptide were increased at all stages of the disease. Conclusion: Activation of lipid peroxidation and catalase can be considered as an additional criterion for evaluating the degree of antioxidant defense in patients with oxalate urolithiasis.