Endogenous intoxication associated with anesthetic preconditioning in hemorrhagic hypotension

Abstract

Background. Interest in the possibility of reducing endogenous intoxication in acute massive blood loss is growing. The phenomenon of anesthetic preconditioning has been actively discussed since a possibility of additional protection of various organs and systems has appeared.
The aim of this study was to assess parameters of endogenous intoxication associated with anesthetic preconditioning with sevoflurane in hemorrhagic hypotension.
Materials and methods. Experiments were carried out on 100 white outbred male rats divided into two control groups (10 intact rats in each group anesthetized with ether or sevoflurane) and two experimental groups (40 rats in each group with hemorrhagic hypotension induced during anesthesia with ether or sevoflurane). After 15, 30, 60, and 120 min of hemorrhagic hypotension, the blood content of low and medium molecular weight substances was measured in the blood from the common carotid artery of control and experimental rats. The peptide-nucleotide coefficient and the aromaticity coefficient were calculated for qualitative assessment of the substance pool. Statistical analysis was performed with the nonparametric Mann-Whitney test.
Results. Indices of endogenous intoxication of anabolic and catabolic pools were decreased in the groups receiving sevoflurane. The blood peptide-nucleotide coefficient was decreased only at 15 min of hemorrhagic hypotension during sevoflurane anesthesia compared to the experimental group receiving ether.
Conclusions. In control animals anesthetized with sevoflurane for anesthesia, systemic endotoxemia was less pronounced than in the diethyl ether group. In hemorrhagic hypotension, indexes of endogenous intoxication were lower for sevoflurane anesthesia, which evidenced systemic cytoprotection, and, thus, occurrence of the effect of anesthetic preconditioning.