Registration of isotype composition of immune complexes as a new diagnostic procedure

  • S B Landa B.P. Konstantinov Peterburg Nuclear Phisics Institute NRC «Kurchatov Institute» Gatchina
  • A V Ivanov University Hospital of Sait-Petersburg State University Sait-Petersburg
  • E V Komlichenko University Hospital of Sait-Petersburg State University Sait-Petersburg
  • M V Filatov B.P. Konstantinov Peterburg Nuclear Phisics Institute NRC «Kurchatov Institute» Gatchina
Keywords: Dynamic light scattering, particle size distribution, circulating immune complexes, immunoglobulin

Abstract

In this paper we propose a new approach analysis of circulating immune complexes (CIC) formed in heterogeneous biological fluids. The method is based on an analysis of the contribution of immune complexes in light scattering that is logged by using dynamic light scattering spectrometer with heterodyne measurement scheme [4]. The proposed approach is capable of detecting immune complexes formed by the addition of the antigen in a concentration greater than 50 pg per ml. Increased immune complexes due to their size further aggregation using antibodies specific for the various human immunoglobulin isotype allows to determine the heterogeneity of the composition and complexes.

Published
2017-12-30
How to Cite
Landa, S. B., Ivanov, A. V., Komlichenko, E. V., & Filatov, M. V. (2017). Registration of isotype composition of immune complexes as a new diagnostic procedure. Patogenez (Pathogenesis), 13(4), 51-56. Retrieved from https://pathogenesis.pro/article/view/43
Issue
Vol. 13 No. 4 (2015): Pathogenesis
Section
Original investigations