Concentration of soluble CD 25 in patients with colorectal cancer
Abstract
Background: Soluble CD25, an interleukin-2 alpha receptor subunit (sIL-2Ra, sSD25), can be considered as a diagnostic marker that predicts the disease course and outcome in patients with malignant tumors.
Aim: To assess the concentration of CD25 in blood serum and tumor tissue in patients with colorectal cancer (CRC).
Materials and methods: The study enrolled 44 patients with colorectal cancer and 25 patients with benign colon tumors. The control group consisted of 25 patients who had been operated for colon injury. Concentrations of sCD25 were measured in blood serum and in the supernatant of tumor tissue (intestinal tissue in control) and lymph node homogenates by flow cytometry on a CytoFlex LX analyzer (Beckman Coulter, USA) with a LEGENDplex ™ HU multiplex analysis kit (Immune Checkpoint, USA). Significance of differences was determined with the nonparametric Mann-Whitney U test.
Results: High concentrations of sCD25 were observed in both patient groups with large intestinal neoplasms, but they were higher in patients with colorectal cancer. In patients with colon cancer, the serum concentration of sCD25 was increased by 6.9 [5.21; 8.73] times compared to the control group (p < 0.001) and by 1.59 [1.36; 2.36] times (p = 0.003) compared to patients with benign tumors. The tumor tissue concentration of sCD25 was 6.75 [5.73; 8.73] times higher in CRC patients than in the control group (p < 0.001) and 2.06 [1.60; 2.52] times higher than in patients with benign colon tumor (p = 0.001). The lymph node tissue concentration of sCD25 was 1828.6 [1777.0; 3121.8] pg/ml in patients with colorectal cancer. It was found that a serum concentration of sCD25 higher than 85.9 pg/ml was associated with a high probability of large intestinal oncology. The serum concentration of sCD25 relatively strongly correlated with its tissue concentration.
Conclusion: The study showed than serum and tissue concentrations of sCD25 were increased in patients with colon neoplasms compared to the control group. The highest sCD25 concentrations were observed in patients with colorectal cancer.
