Highly sensitive AFM-fishing of albumin

  • T. O. Pleshakova Institute of Biomedical Chemistry, Moscow, Russia https://orcid.org/0000-0003-3217-9095
  • K. A. Malsagova Institute of Biomedical Chemistry, Moscow, Russia
  • A. F. Kozlov Institute of Biomedical Chemistry, Moscow, Russia
  • S. L. Kanashenko Institute of Biomedical Chemistry, Moscow, Russia
  • N. D. Ivanova Moscow state academy of veterinary medicine and biotechnology named after K.I. Skryabin, Moscow, Russia
  • R. A. Galiullin Institute of Biomedical Chemistry, Moscow, Russia
  • T. A. Sadovskaya Moscow state academy of veterinary medicine and biotechnology named after K.I. Skryabin, Moscow, Russia
  • A. I. Archakov Institute of Biomedical Chemistry, Moscow, Russia
  • Yu. D. Ivanov Institute of Biomedical Chemistry, Moscow, Russia https://orcid.org/0000-0001-5041-1914
Keywords: AFM, the detection of low-abundance proteins, fishing proteins

Abstract

This paper proposes a method of highly sensitive atomic-force microscopy (AFM) detection of human serum albumin (HAS) molecules using AFM fishing with charge generation. Our results indicate that injection of water or protein solution with the rate of about 1 ml/sec causes charge generation within the measuring cell due to triboelectric effect with the rate of about 1 nC/sec. Moreover, it is connected with the effec tive protein fishing onto highly oriented pyrolytic graphite (HOPG) surface of the AFM chip with a limit of detection (LOD) of as low as 10-18 M. Decrease of solution injection rate leads to decrease of fishing effectiveness. It is also demonstrated that charge generation rates caused by triboelectric effect and by external voltage of 10kV are similar. The method of AFM fishing thus has demonstrated an immense potential as a method of creating medical diagnostic devices and instruments.

Published
2016-09-01
How to Cite
Pleshakova, T. O., Malsagova, K. A., Kozlov, A. F., Kanashenko, S. L., Ivanova, N. D., Galiullin, R. A., Sadovskaya, T. A., Archakov, A. I., & Ivanov, Y. D. (2016). Highly sensitive AFM-fishing of albumin. Patogenez (Pathogenesis), 14(3), 23-30. Retrieved from https://pathogenesis.pro/article/view/68
Issue
Vol. 14 No. 3 (2016): Pathogenesis
Section
Original investigations