Способ определения циркулирующих иммунных комплексов

B. B. Shoibonov; V. Yu. Baronets; L. F. Panchenko; A. A. Kubatiev

B. B. Shoibonov Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; National Research Center for Addictions Russian Ministry of Public Health, Moscow, Russian Federation; Anokhin Institute of Normal Physiology, RAMS, Moscow, Russian Federation
V. Yu. Baronets Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; National Research Center for Addictions Russian Ministry of Public Health, Moscow, Russian Federation
L. F. Panchenko Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; National Research Center for Addictions Russian Ministry of Public Health, Moscow, Russian Federation
A. A. Kubatiev Institute of General Pathology and Pathophysiology, Moscow, Russian Federation

Keywords: circulating immune complexes, polyethylene glycol 3350, complement system, pathogenicity of the CIC

Abstract

There has been developed a simple method for determining circulating immune complexes (CIC), based on their precipitation from human serum in a special buffer containing 10% polyethylene glycol mol. weight 3350 (PEG-3350). We add human serum to an equal volume of buffer containing 10% PEG-3350, incubated for 10 min at room temperature. CIC aggregated by centrifugation, are dissolved in buffer without PEG-3350, and we determine the total level of the CIC or by spectrophotometry at 280 nm and 260 nm, or the determination of protein in the precipitate is performed according to the method of Lowry. The method improves the accuracy of the quantitative determination of both general (CICtot) and complement-binding (CIC-1) and non-binding (CIC-2) circulating immune complexes in human serum. Pathogenicity of the CIC is calculated as the ratio of the CIC-1 to CIC-2.

Method for determining circulating immune complexes

Abstract