The effect of the pharmacological model of intestinal microbiota disorders on the behavioral reactions of rats

Abstract

The aim of the study was to study the effect of the pharmacological model of intestinal microbiota disorders on the behavioral reactions of adult rats in various test facilities

Materials and methods. The study was performed on adult male rats of the Wistar line in compliance with the norms of bioethics. Rats of the experimental group (n = 6) daily for 10 days A complex suspension of various antibiotics was administered per os day (vancomycin, 0.5 g/kg; metronidazole 0.5 g/kg; ampicillin, 0.5 g/kg). The rats of the control group (n = 6) received sterile water according to a similar scheme. The specific and quantitative analysis of the intestinal microbiota was carried out using methods of fecal mass seeding, light microscopy and mass- spectrometry. Behavioral reactions were studied using various tests (open field, raised cruciform maze, Barnes maze). The specific and quantitative composition of the intestinal microbiota, behavioral reactions were analyzed in the initial state and 10 days after administration of the substances. The results of the study were processed using the static program SigmaStat 12.5. Statistically significant results were considered at p < 0.05.

Results. The action of the antibiotic complex led to significant depletion and a change in the composition of obligate intestinal microbes in rats, as evidenced by the results microscopic analysis of the seed material and mass spectrometry data. In particular, there was a decrease in the number of lactobacilli, micrococci, enterococci and E. coli. The predominance of S. maltophilia was observed in the crops. The pharmacological model of intestinal microbiota disorders led to changes in the behavioral reactions of rats in various tests. In the open However, under the influence of the antibiotic complex, there was a decrease in research activity. Exposure to antibiotics increased the level of anxiety, as evidenced by a decrease in the time spent by rats in the open arms of the cruciform maze. In the Barnes maze, the administration of a complex of antibiotics had a negative effect on the spatial memory of rats.

Conclusion. Exposure to a cocktail of antibacterial drugs causes a violation of the intestinal microbiota in rats, associated with a progressive decrease in research activity and spatial memory function against the background of increased anxiety.