Circulating stem and progenitor cells as markers of inflammation, endothelial regeneration, and prediction of vascular complications in metabolic syndrome and type 1 and 2 diabetes mellitus

  • O. V. Pershina E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • A. V. Pakhomova E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • N. N. Ermakova E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • O. Yu. Rybalkina E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • V. A. Krupin E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • E. S. Pan E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • O. E. Vaizova Siberian State Medical University, Tomsk, Russia
  • A. I. Kravchenko Open Laboratory Ltd, Tomsk, Russia
  • Yu. G. Samoilova Siberian State Medical University, Tomsk, Russia
  • M. A. Rotkang Siberian State Medical University, Tomsk, Russia
  • A. M. Dygai E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
  • E. G. Skurikhin E.D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
DOI: https://doi.org/10.25557/2310-0435.2018.01.58-67
Keywords: endothelial progenitor cells, mesenchymal stem cells, hematopoietic stem cells, diabetes, metabolic syndrome, vascular complications

Abstract

The aim of this study was to identify mesenchymal stem cells (MSC), hematopoietic stem cells (HSC), mature endothelial cells, and endothelial progenitor cells (EPC) in the blood of healthy volunteers, patients with metabolic syndrome, and type 1 and 2 diabetes mellitus as new, informative cellular markers of vascular complications, endothelial regeneration, and inflammation. Methods. The diagnosis was confirmed by general clinical, biochemical, coagulometeric and ELISA studies; multi-parameter cytometric assay was used for evaluation of antigen expression. Results. Changes in the count of MSC, HSC, mature endothelial cells, and endothelial progenitor cells in blood of patients with metabolic syndrome and type 1 and 2 diabetes depended on the type of pathology. We propose using endothelial cells of myeloid (CD45-CD14+CD34+CD309+CD144+CD31+) and non-myeloid origin (CD45-CD14-CD34+CD309+CD144+CD31+), CD309+-endothelial cells, and MSCs with the
CD44+CD73+CD90+CD105+ phenotype as nonspecific markers of endothelial damage in presence of diabetic symptoms. Furthermore, HSCs (CD45+CD34+) can be used as a valuable diagnostic and prognostic marker of inflammation. Conclusions. It is relevant to evaluate EPCs of non-bone marrow localization (CD31+CD309+CD144+) and bone marrow localization (CD34+CD309+) and EPCs with a high regenerative potential
(CD45-CD34+CD31+CD144+) in the blood of patients with type 1 and 2 diabetes to confirm the presence of vascular damage and predict development of complications. Circulating, in vitro colony-forming EPCs (CD45-CD34+CD31+) are recommended as a differential marker for inhibition of endothelial regeneration in type 2 diabetes.

Published
2018-03-20
How to Cite
Pershina, O., Pakhomova, A., Ermakova, N., Rybalkina, O., Krupin, V., Pan, E., Vaizova, O., Kravchenko, A., Samoilova, Y., Rotkang, M., Dygai, A., & Skurikhin, E. (2018, March 20). Circulating stem and progenitor cells as markers of inflammation, endothelial regeneration, and prediction of vascular complications in metabolic syndrome and type 1 and 2 diabetes mellitus. Patogenez (Pathogenesis), 16(1), 58-67. https://doi.org/https://doi.org/10.25557/2310-0435.2018.01.58-67
Issue
Vol 16 No 1 (2018): Pathogenesis
Section
Clinical researches