The role of miRNA in regulation of AXL, DAPK1, NFIB gene expression in breast cancer

  • I. V. Pronina Institute of General Pathology and Pathophysiology, Moscow, Russia http://orcid.org/0000-0002-0423-7801
  • E. A. Filippova Institute of General Pathology and Pathophysiology, Moscow, Russia http://orcid.org/0000-0001-7172-0433
  • S. C. Lukina I.M. Sechenov First Moscow State Medical University, Moscow, Russia
  • A. M. Burdenny Institute of General Pathology and Pathophysiology, Moscow, Russia
  • T. P. Kazubskaya N.N. Blokhin National Medical Research Center of Oncology, Moscow, Russia
  • E. A. Braga Institute of General Pathology and Pathophysiology, Moscow, Russia; Medical Genetics Research Center, Moscow, Russia
  • V. I. Loginov Institute of General Pathology and Pathophysiology, Moscow, Russia http://orcid.org/0000-0003-2668-8096
DOI: https://doi.org/10.25557/2310-0435.2018.04.130-133
Keywords: breast cancer, miRNA, mRNA, apoptosis

Abstract

Breast cancer (BC) is characterized by epigenetic disorders, which lead to dysregulation of protein-coding gene expression; together these result in development of a tumor. The goal of the study was to search for new miRNAs that are potentially involved in regulation of the expression of three protein-encoding genes (AXL, DAPK1, NFIB) associated with regulation of apoptosis and the epithelial-mesenchymal transition in breast cancer. Quantitative PCR was used to determine changes in the expression of three protein-coding genes (AXL, DAPK1, NFIB) and three miRNAs (miR-127-5p, -132-3p, -9-5p) that had been predicted to be regulators by miRWalk 2.0 algorithms. Statistically significant negative correlations between changes in miRNA and mRNA expression were determined for the following pairs: miR-127-5p – DAPK1 (Rs = –0.503, p = 0.001) and miR-9-5p – DAPK1 (Rs = –0.335, p = 0.040). Therefore, the study showed a potential role of two miRNAs in regulation of the DAPK1 gene expression, an activator of various apoptotic pathways and a negative regulator of EMT. This result is fundamentally important and can be used to develop targeted therapies for breast cancer.

Published
2018-12-11
How to Cite
Pronina, I., Filippova, E., Lukina, S., Burdenny, A., Kazubskaya, T., Braga, E., & Loginov, V. (2018, December 11). The role of miRNA in regulation of AXL, DAPK1, NFIB gene expression in breast cancer. Patogenez (Pathogenesis), 16(4), 130-133. https://doi.org/https://doi.org/10.25557/2310-0435.2018.04.130-133
Issue
Vol 16 No 4 (2018): Pathogenesis
Section
Brief reports

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