Effect of inhalation of tobacco smoke on the phenotype alveolar macrophages mice of different genetic lines

Abstract

The defining role of tobacco smoking as a risk factor in the development of chronic obstructive pulmonary diseases (COPD) is of no doubt today. We performed in vivo experimental modeling of COPD in mice of different genetic strains (С57 and BALB/c). We studied phenotypes of the main cells of innate immunity system — alveolar macrophages and revealed genetic and age predispositions in COPD development in experimental animals. It was shown: 1) there is a certain genetic predisposition to the influence of tobacco smoke, probably, associated with proinflammatory М1 phenotype of macrophages; 2) aging promotes macrophages phenotype transformation towards antiinflammatory М2 which is more expressed in macrophages of mice with initially genetically determined М1 phenotype (mice strain С57), and 3) long inhalation of tobacco smoke enhances age-dependent transformation of macrophages aside М2 phenotype, that is also more expressed in macrophages of mice with genetically determined М1 phenotype (С57).