5НТ1В- и 5НТ2В-рецепторы вызывают увеличение концентрации ионов кальция в эндотелиальных клетках кровеносных сосудов

I. L. Terekhina; A. D. Nadeev; L. M. Kozhevnikova; N. V. Goncharov; P. V. Aldonin

I. L. Terekhina Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
A. D. Nadeev Research Institute of Hygiene, Occupational Pathology and Human Ecology, FMBA of Russia, St. Petersburg, Russian Federation
L. M. Kozhevnikova Institute of General Pathology and Pathophysiology, Moscow, Russian Federation
N. V. Goncharov Research Institute of Hygiene, Occupational Pathology and Human Ecology, FMBA of Russia, St. Petersburg, Russian Federation
P. V. Aldonin Institute of General Pathology and Pathophysiology, Moscow, Russian Federation; Federal State Budgetary Institution Institute of Developmental Biology named after N.K. Koltsov RAS, Moscow, Russian Federation

Keywords: serotonin, 5HT1B- and 5HT2B-receptors, endothelial cells, aorta, calcium

Abstract

The aim of the work was to study the mechanism of vasodilatation in mediated by 5HT1B- and 5HT2B-receptors of serotonin. The experiments were performed on isolated rat aorta and on cultured endothelial cells (EC) from human pulmonary vein and smooth muscle cells (SMC) from rat aorta. CGS12066B and BW723C were used for the activation of 5HT1B- and 5HT2B-receptors respectively. It was demonstrated that CGS12066B and BW723C induced elevation of free cytoplasmic calcium concentration ([Ca²⁺]_cyt) in EC. These effects ofCGS12066B and BW723C were observed at concentrations at which they dilate aorta. It is concluded that 5HT1B- and 5HT2B-receptors cause vasodilation according endothelium-dependent mechanism based on elevation of [Ca²⁺]_cyt in EC.

5HT1B- and 5HT2B-receptors of serotonin stimulate increase in cytoplasmic calcium concentrations in vascular endothelial cells

Abstract