Polymorphic locus A8202G of the matrix metalloproteinase 9 gene in the development of diabetic foot

Abstract

The aim of the study was to evaluate the frequency of distribution of alleles and genotypes of the A8202G polymorphic marker of the matrix metalloproteinase 9 gene in patients with diabetic foot in the population of the Trans-Baikal Territory.

Materials and methods. The study included 199 patients with type 2 diabetes mellitus and a mixed form of diabetic foot syndrome, 198 patients with type 2 diabetes mellitus and 100 healthy individuals, in whom the frequency of distribution of the genotypes of the A8202G polymorphic marker of the matrix metalloproteinase 9 gene was studied by polymerase chain reaction.

Results. The distribution frequency of the genotypes of the A8202G polymorphic marker of the matrix metalloproteinase 9 gene and allelic variants in the group with diabetic foot, diabetes mellitus and the control group did not differ significantly. The A/A genotype in patients with diabetic foot syndrome was detected only 20% more often than in the control group, and 20% less often than in patients with diabetes mellitus (differences are not statistically significant). The A/G genotype of the A8202G polymorphic marker of the MMP9 gene was registered in the group of diabetic foot patients by 20% and 10% less frequently than in the control and diabetic groups, respectively (differences were not statistically significant). The frequencies of the G/G genotype of the A8202G polymorphic marker of the MMP9 gene in the groups with diabetic foot syndrome and control also did not differ and amounted to 37.0% and 37.2%, respectively. The specified genotype of the A8202G polymorphism of the studied gene in the development of DFS was more common than in DM, by 30%. Allele A of the polymorphic marker A8202G of the MMP9 gene in the development of DFS was detected with a frequency of 0.549, in healthy individuals – with a frequency of 0.435, with DM – with a frequency of 0.492. The G allele frequency in the group of patients was 0.451, in the control group – 0.565, in the group with DM – 0.508.

Conclusion: in the population of the Trans-Baikal Territory in patients with type 2 diabetes mellitus, there is no relationship between genotype variants and alleles of the A8202G polymorphic marker of the MMP9 gene with the risk of developing diabetic foot syndrome.