Reactive microglia in intraperitoneal administration of lipopolysaccharide

Abstract

The aim of study was to reveal the expression of the marker of activated Iba1 macrophages in the cingulate cerebral cortex of mice when escherichial lipopolysaccharide (LPS) given.

Materials and methods. The work included three-month-old female C57Bl/6 mice. Animals of Group 1 got intraperitoneal saline solution once a day for 4 days, Group 2 – 0.5 mg/kg/day of E. coli LPS, and Group 3 – 2 mg/kg of the substance. Mice sacrificed, brain sections of 5 µm made, and Iba1-antibodies’ staining performed with consecutive morphometrics and statistics.

Results. The mean number of sites with a positive reaction to Iba1 in Group 3 is significantly higher than that for Group 1, p <0.05.

Conclusion. LPS i. p. at 2 mg/kg/day for 4 days leads to an increase in Iba1 expression in the cingulate cerebral cortex of mice. Further study of this mode of LPS administration is required.